Fraser Broth Base is used with Acriflavin, Nalidixic Acid, and Ferric Ammonium Citrate for the selective enrichment of Listeria spp. Fraser Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Formulated according to MLG Appendix 1.09 (for the ISO 11290 formulation please use FSA1268/FSA1270). Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicated the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurised milk, Mexican style cheese and pate.

Half Fraser (Demi Fraser) Broth Base is based on the formulation of Fraser and Sperber. This medium is used in rapid detection of Listeria from food and environmental samples. Listeria spp. grow over a pH range of 5.0 - 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria spp. is based on successful isolation of the organism, biochemical characterisation, and serological confirmation.

• Analyte: Listeria spp.
• Platform: dehydrated culture media
• Formula: proteose peptone 5.0g/L, tryptone 5.0g/L, beef extract 5.0g/L, yeast extract 5.0g/L, esculin 1.0g/L, disodium hydrogen phosphate 12.0g/L, potassium dihydrogen phosphate 1.35g/L, sodium chloride 20.0g/L, lithium chloride 3.0g/L
• Grams per litre: 57.40
• Litres per 500g: 9.00